Quantitative comparison of membrane transduction and endocytosis of oligopeptides.


A novel method was developed to quantitatively determine the membrane transduction efficiency of cationic oligopeptides, allowing for selective measurement of transduction, excluding concurrent adsorptive endocytosis. It was found that Tat-(47-57) (YGRKKRRQRRR), YG(l-R)(9), and guanidinated-YG(l-K)(9) were preferentially transduced to the cytosolic… (More)