Quantitative comparison of insulin-like growth factor mRNA levels in human and rat tissues analysed by a solution hybridization assay.

@article{Mller1991QuantitativeCO,
  title={Quantitative comparison of insulin-like growth factor mRNA levels in human and rat tissues analysed by a solution hybridization assay.},
  author={Christine M{\"o}ller and Peter Arner and Tomas Sonnenfeld and Gunnar Norstedt},
  journal={Journal of molecular endocrinology},
  year={1991},
  volume={7 3},
  pages={213-22}
}
In this study a solution hybridization assay was evaluated for its application to the measurement of levels of specific mRNAs. The evaluation included parameters such as incubation time, hybridization stringency and probe concentration/structure. Both short (50 bases derived from synthetic oligonucleotides) and long (125-147 bases) RNA probes, derived from cloned sequences, could be used to obtain quantitative information on specific mRNA species. The solution hybridization assay was used to… CONTINUE READING