Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.

@article{PadillaParra2009QuantitativeCO,
  title={Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.},
  author={Sergi Padilla-Parra and Nicolas Audug{\'e} and Herv{\'e} Lalucque and Jean-Claude eLe M{\'e}vel and Ma{\"i}t{\'e} Coppey-Moisan and Marc Tramier},
  journal={Biophysical journal},
  year={2009},
  volume={97 8},
  pages={
          2368-76
        }
}
The fluorescent-protein based fluorescence resonance energy transfer (FRET) approach is a powerful method for quantifying protein-protein interactions in living cells, especially when combined with fluorescence lifetime imaging microscopy (FLIM). To compare the performance of different FRET couples for FRET-FLIM experiments, we first tested enhanced green fluorescent protein (EGFP) linked to different red acceptors (mRFP1-EGFP, mStrawberry-EGFP, HaloTag (TMR)-EGFP, and mCherry-EGFP). We… CONTINUE READING
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