Quantitative comparison between poly(L-arginine) and poly(L-lysine) at each step of polyplex-based gene transfection using a microinjection technique.

@article{Hashimoto2012QuantitativeCB,
  title={Quantitative comparison between poly(L-arginine) and poly(L-lysine) at each step of polyplex-based gene transfection using a microinjection technique.},
  author={Tomoko Hashimoto and Takeshi Kawazu and Takeshi Nagasaki and Akira Murakami and Tetsuji Yamaoka},
  journal={Science and technology of advanced materials},
  year={2012},
  volume={13 1},
  pages={015009}
}
Among the well-studied polypeptide-type gene carriers, transfection efficiency is empirically known to be higher for poly(L-arginine) (PR) than poly(L-lysine) (PK). The big difference between PR and PK should be determined at one of the intracellular trafficking steps based on the different charge densities, structures or PKa values. However, the endosomal escape and the intranuclear transcription efficiency in living cells have not been clarified yet. In this study, a novel method for… CONTINUE READING

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