Quantitative colocalization analysis of fluorescence microscopy images.

@article{Zinchuk2014QuantitativeCA,
  title={Quantitative colocalization analysis of fluorescence microscopy images.},
  author={Vadim S. Zinchuk and Olga Grossenbacher-Zinchuk},
  journal={Current protocols in cell biology},
  year={2014},
  volume={62},
  pages={
          Unit 4.19.1-14
        }
}
Colocalization is an important finding in many cell biological studies. This unit describes a protocol for quantitative evaluation of fluorescence microscopy images with colocalization based on calculation of a number of specialized coefficients. Images of double-stained sections are first subjected to background correction, and then various coefficients are calculated. Meanings of the coefficients and a guide to interpretation of the results of calculations based on the use of linguistic… CONTINUE READING
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