Quantitative characterization of yeast cell incorporation in phagocytes: microscopic methods for differentiation between adherent and ingested particles.

@article{Sthl1989QuantitativeCO,
  title={Quantitative characterization of yeast cell incorporation in phagocytes: microscopic methods for differentiation between adherent and ingested particles.},
  author={Morgan St{\aa}hl and K Reifenberg and Uli L{\"o}sch},
  journal={Zentralblatt fur Veterinarmedizin. Reihe B. Journal of veterinary medicine. Series B},
  year={1989},
  volume={36 3},
  pages={
          185-90
        }
}
A light- and a fluorescence-microscopic method for quantitative assessment of yeast cell incorporation in phagocytes were developed. The light-microscopic method offers methylene blue prestained yeast cells as phagocytosis particles and counterstains nonincorporated yeasts with eosine. The fluorescence-microscopic method works by acridine orange staining of phagocytosis assays. Fluorescence of nonincorporated yeast cells is suppressed by addition of methylene blue. Different ways of evaluating… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.

Self-regulation of neutrophils during phagocytosis is modified after severe tissue injury.

  • International journal of molecular medicine
  • 2006
VIEW 4 EXCERPTS
CITES BACKGROUND & METHODS
HIGHLY INFLUENCED