Quantitative caveats of standard immunohistochemical procedures: implications for optical disector-based designs.


Immunohistochemistry is a ubiquitous technique in histology. Often, the goal of such studies is the quantification of some parameter associated with a particular antigen. When used correctly, the optical disector offers a statistically relevant approach to achieve this goal without bias from cell size, shape, or orientation. This three-dimensional counting… (More)
DOI: 10.1369/jhc.2009.954164


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