Quantitative autoradiography of serotonin uptake sites in rat brain using [3H]cyanoimipramine

@article{Kovachich1988QuantitativeAO,
  title={Quantitative autoradiography of serotonin uptake sites in rat brain using [3H]cyanoimipramine},
  author={Gyula B. Kovachich and Carl E. Aronson and David J. Brunswick and Alan Frazer},
  journal={Brain Research},
  year={1988},
  volume={454},
  pages={78-88}
}
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References

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TLDR
The results indicate that [3H]CN-IMI can be given to rats to provide a measure of serotonin uptake sites in the central nervous system in vivo and show that the binding in vivo was saturable.
Selective labeling of serotonin uptake sites in rat brain by [3H]citalopram contrasted to labeling of multiple sites by [3H]imipramine.
TLDR
Inhibition of [3H]citalopram binding by both serotonin and imipramine is consistent with a competitive interaction in both equilibrium and kinetic analyses, and the regional distribution of serotonin sensitive and serotonin-insensitive high affinity binding sites closely resembles that of citalopam binding.
Does high affinity [3H] imipramine binding label serotonin reuptake sites in brain and platelet?
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TLDR
The anatomical distribution of binding sites demonstrated is consistent with the specific labelling of 5‐HT neurons by [3H]indalpine and confirms previous studies carried out with another 5-HT uptake inhibitor, [3 H]imipramine.
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TLDR
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Quantitative Autoradiography of [3H]Indalpine Binding Sites in the Rat Brain: I. Pharmacological Characterization
TLDR
Electrolytic lesions of the dorsal raphe or medial forebrain bundle, which cause a degeneration of 5‐HT cell bodies and fibers, respectively, resulted in a 30–40% reduction in the binding of [3H]indalpine.
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TLDR
A nonpeptidic constituent of rat brain capable of displacing [3H]imipramine from its high-affinity binding site and of inhibiting the serotonin uptake in a dose-related manner has been extracted and its partial purification is described.
Quantitative autoradiographic localization of [3H]imipramine binding sites in the brain of the rat: relationship to ascending 5-hydroxytryptamine neuron systems.
TLDR
The present results underline the possibility that the 5-HT and dopamine hypotheses for the mechanism of action of antidepressant drugs are not mutually exclusive, because both 5- HT and dopamine neurons can be regulated by large numbers of [3H]imipramine binding sites.
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