Quantitative autoradiography of serotonin uptake sites in rat brain using [3H]cyanoimipramine

@article{Kovachich1988QuantitativeAO,
  title={Quantitative autoradiography of serotonin uptake sites in rat brain using [3H]cyanoimipramine},
  author={Gyula B. Kovachich and Carl E. Aronson and David J. Brunswick and Alan Frazer},
  journal={Brain Research},
  year={1988},
  volume={454},
  pages={78-88}
}
[3H] sertraline binding to rat brain membranes
TLDR
A marked decrease in ex vivo [3H] sertraline binding in the brain of rats 7 days after treatment withp-chloroamphetamine was consistent with the loss of serotonin uptake sites induced by this agent.
In Vivo Labeling of Serotonin Uptake Sites with [3H]Paroxetine
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There was an excellent correlation between the in vivo inhibitory potencies of serotonin uptake blockers in this study and previously published in vitro data on inhibition of [3H]serotonin uptake in brain synaptosomes.
Quantitative autoradiography of 3H-paroxetine binding sites in rat brain.
Validation of the transporter ligand cyanoimipramine as a marker of serotonin innervation density in brain.
TLDR
Under drug-free conditions, specific [3H]CYI binding is a good quantitative index of 5- HT innervation density in brain tissue and is not significantly up- or downregulated on 5-HT denervation or hyperinnervation.
Quantitative radioluminography of serotonin uptake sites in the porcine brain
TLDR
The findings show that the anatomic distribution of serotonin uptake sites in the porcine brain is similar to that reported in other mammals, and the density was close to those reported in human brain and in rat brain.
Binding of some antidepressants to the 5-hydroxytryptamine transporter in brain and platelets
TLDR
Antidepressant agents with properties to inhibit 5-hydroxytryptamine uptake in brain tissue and platelets bind with high affinities to neuronal and platelet membranes and appear to be better ligands than [3H]imipramine.
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TLDR
The results indicate that [3H]CN-IMI can be given to rats to provide a measure of serotonin uptake sites in the central nervous system in vivo and show that the binding in vivo was saturable.
Selective labeling of serotonin uptake sites in rat brain by [3H]citalopram contrasted to labeling of multiple sites by [3H]imipramine.
TLDR
Inhibition of [3H]citalopram binding by both serotonin and imipramine is consistent with a competitive interaction in both equilibrium and kinetic analyses, and the regional distribution of serotonin sensitive and serotonin-insensitive high affinity binding sites closely resembles that of citalopam binding.
Quantitative Autoradiography of [3H]Indalpine Binding Sites in the Rat Brain: II. Regional Distribution
TLDR
The anatomical distribution of binding sites demonstrated is consistent with the specific labelling of 5‐HT neurons by [3H]indalpine and confirms previous studies carried out with another 5-HT uptake inhibitor, [3 H]imipramine.
Single-site model of the neuronal 5-hydroxytryptamine uptake and imipramine-binding site.
TLDR
Both displacement and saturation studies in which two displacing agents were combined indicated that most of the binding competed by 5-HT and norzimeldine is identical and is subsumed within 6-MeO-TH beta C (30 microM)-displaceable binding.
Quantitative Autoradiography of [3H]Indalpine Binding Sites in the Rat Brain: I. Pharmacological Characterization
TLDR
Electrolytic lesions of the dorsal raphe or medial forebrain bundle, which cause a degeneration of 5‐HT cell bodies and fibers, respectively, resulted in a 30–40% reduction in the binding of [3H]indalpine.
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TLDR
A nonpeptidic constituent of rat brain capable of displacing [3H]imipramine from its high-affinity binding site and of inhibiting the serotonin uptake in a dose-related manner has been extracted and its partial purification is described.
Quantitative autoradiographic localization of [3H]imipramine binding sites in the brain of the rat: relationship to ascending 5-hydroxytryptamine neuron systems.
TLDR
The present results underline the possibility that the 5-HT and dopamine hypotheses for the mechanism of action of antidepressant drugs are not mutually exclusive, because both 5- HT and dopamine neurons can be regulated by large numbers of [3H]imipramine binding sites.
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