Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche)

@article{Ajubi2005QuantitativeAH,
  title={Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche)},
  author={Nasser E. Ajubi and Nine Nijholt and Albert M. Wolthuis},
  journal={Clinical Chemistry and Laboratory Medicine (CCLM)},
  year={2005},
  volume={43},
  pages={68 - 70}
}
Abstract Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra… Expand
Performance of qualitative urinary hCG assays
Abstract Significant differences in the sensitivity of eight frequently used qualitative urine human chorionic gonadotropin (hCG) tests in Belgium were observed in this study. Although mostExpand
The need for a quantitative urine hCG assay.
TLDR
The need for a quantitative urine hCG assay is undeniable and manufacturers are invited to produce a quantitative kidney chorionic gonadotropin test. Expand
Estimating the hCGβcf in urine during pregnancy.
TLDR
A correlation exists between serum and urine hCG concentrations but this correlation is variable and hCGβcf concentrations can be estimated using two automated assay reagent platforms that differ in their recognition of hCG βcf. Expand
Quantification of urinary chorionic gonadotropin in spontaneous abortion of pre-clinically recognized pregnancy: method development and analytical validation.
TLDR
The development and validation procedures of an automated solid-phase two-site chemiluminescent immunometric assay for the quantification of urinary hCG in early pregnancy and early pregnancy loss are described and it can be concluded that urinary h CG is stable under the expected conditions required for ongoing investigations. Expand
The analytical specificity of human chorionic gonadotropin assays determined using WHO International Reference Reagents.
BACKGROUND Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone with considerable molecular heterogeneity. There is uncertainty regarding which hCG variants are detected byExpand
Qualitative point-of-care and over-the-counter urine hCG devices differentially detect the hCG variants of early pregnancy.
TLDR
Both OTC and POC devices are capable of detecting hCG concentrations in early pregnancy urine, and OTC devices demonstrated better analytical sensitivity relative to Poc devices. Expand
The potential use of urinary hCG measurements in the management of pregnancies of unknown location.
TLDR
Paired urine and serum hCG measurements differed quite widely but were well correlated and the degree of correlation improved after creatinine correction, and serial urine h CG measurements may have a role in identifying subtypes of low-risk PUL. Expand
Falsely decreased human chorionic gonadotropin (hCG) results due to increased concentrations of the free beta subunit and the beta core fragment in quantitative hCG assays.
TLDR
Extremely high concentrations of hCG variants can cause falsely decreased results in certain quantitative hCG assays, and two assays exhibited decreased hCG results in the presence of h CGβcf concentrations found during normal pregnancy. Expand
Human chorionic gonadotropin tests
  • L. Cole
  • Medicine
  • Expert review of molecular diagnostics
  • 2009
TLDR
Human chorionic gonadotropin tests are essential in managing gestational trophoblastic diseases, whether hydatidiform mole, invasive mole or choriocarcinoma, and are very useful in management of testicular malignancy and other germ cell malignancies. Expand
Human chorionic gonadotropin and associated molecules
  • L. Cole
  • Medicine
  • Expert review of molecular diagnostics
  • 2009
TLDR
Human chorionic gonadotropin tests are essential in managing gestational trophoblastic diseases, whether hydatidiform mole, invasive mole or choriocarcinoma, and are very useful in management of testicular malignancies and other germ cellmalignancies. Expand
...
1
2
...

References

SHOWING 1-8 OF 8 REFERENCES
Total β-Human Chorionic Gonadotropin Measured in Urine by an Automated Method
TLDR
The present study has validated the Abbott AxSYM Total β-hCG assay (approved for serum use only) for use in the quantitative determination of urinary concentrations of total β- hCG. Expand
Method evaluation for total beta-human chorionic gonadotropin using urine and the ADVIA Centaur.
TLDR
The study of the ADVIA Centaur® (Bayer Corporation) total-hCG (ThCG) serum assay with urine found recovery of hCG (US Pharmaco-peia) added to urine was >100% at all concentrations. Expand
Immunoassay of human chorionic gonadotropin, its free subunits, and metabolites.
  • L. Cole
  • Biology, Medicine
  • Clinical chemistry
  • 1997
TLDR
The structure of h CG and related molecules is reviewed, and recognition of hCG-related molecules by different types of immunoassay is examined. Expand
Human chorionic gonadotropin in cancer.
TLDR
Treatment of relapsing choriocarcinomas and testicular germ cell tumors is often initiated on the basis of rising hCG levels even in the absence of clinical or histological evidence of a relapse. Expand
Free Human Chorionic Gonadotropin β Subunit in Gonadal and Nongonadal Neoplasms
TLDR
The detection of the free hCGβ subunit in serum of nonpregnant subjects was highly diagnostic of malignancy in general and specifically defines a subgroup of aggressive nongonadal malignancies. Expand
The deactivation of hCG by nicking and dissociation.
TLDR
It is concluded that nicking is more prevalent after the hCG peak (after 2 months of pregnancy) and the stabilities of nicked and intact hCG molecules are examined. Expand
Analysis of hCG: Clinical Applications and Assay Requirements
Study of the glycoprotein hormones, including hCG, is complex and evolving, and has benefited from recent major advances in analytical technology and molecular biology. It is important to be aware ofExpand
Total beta-human chorionic gonadotropin measured in urine by an automated method.