Quantitative assessment of picoeukaryotes in the natural environment by using taxon-specific oligonucleotide probes in association with tyramide signal amplification-fluorescence in situ hybridization and flow cytometry.

@article{Biegala2003QuantitativeAO,
  title={Quantitative assessment of picoeukaryotes in the natural environment by using taxon-specific oligonucleotide probes in association with tyramide signal amplification-fluorescence in situ hybridization and flow cytometry.},
  author={Isabelle C. Biegala and Fabrice Not and Daniel Vaulot and Nathalie B. Simon},
  journal={Applied and environmental microbiology},
  year={2003},
  volume={69 9},
  pages={
          5519-29
        }
}
Picoeukaryotes (cells of <3 micro m in diameter) contribute significantly to marine plankton biomass and productivity, and recently molecular studies have brought to light their wide diversity. Among the methods that have been used so far to quantify aquatic microorganisms, fluorescence in situ hybridization of oligonucleotide probes combined with flow cytometry offers the advantages of both high resolution for taxonomic identification and automated cell counting. However, cell losses, cell… CONTINUE READING

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