Quantitative assessment of olfactory receptors activity in immobilized nanosomes: a novel concept for bioelectronic nose.

@article{Vidic2006QuantitativeAO,
  title={Quantitative assessment of olfactory receptors activity in immobilized nanosomes: a novel concept for bioelectronic nose.},
  author={Jasmina Vidic and Jeanne Grosclaude and Marie-Annick Persuy and Josiane Aioun and Roland Salesse and E. Pajot-Augy},
  journal={Lab on a chip},
  year={2006},
  volume={6 8},
  pages={1026-32}
}
We describe how mammalian olfactory receptors (ORs) could be used as sensing elements of highly specific and sensitive bioelectronic noses. An OR and an appropriate G(alpha) protein were co-expressed in Saccharomyces cerevisiae cells from which membrane nanosomes were prepared, and immobilized on a sensor chip. By Surface Plasmon Resonance, we were able to quantitatively evaluate OR stimulation by an odorant, and G protein activation. We demonstrate that ORs in nanosomes discriminate between… CONTINUE READING

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