Quantitative and qualitative in vivo angiogenesis assay.

  title={Quantitative and qualitative in vivo angiogenesis assay.},
  author={Marya F. McCarty and Cheryl Baker and Corazon D. Bucana and Isaiah Josh Fidler},
  journal={International journal of oncology},
  volume={21 1},
We describe the development and optimization of an in vivo angiogenesis assay utilizing gelfoam sponges impregnated with 0.4% agarose and different proangiogenic factors, such as basic fibroblast growth factor (bFGF), vascular epidermal growth factor (VEGF), tumor growth factor-alpha (TGF-alpha), and endothelial growth factor (EGF). The sponges are implanted into the subcutis of mice and harvested after different times. The gelfoam sponges are fixed, sectioned, and stained with fluorescent… CONTINUE READING

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