Quantitative analytical methods for the determination of a new hypertension drug, CGS 25462, and its metabolites (CGS 25659 and CGS 24592) in human plasma by high-performance liquid chromatography.

@article{Maniara1998QuantitativeAM,
  title={Quantitative analytical methods for the determination of a new hypertension drug, CGS 25462, and its metabolites (CGS 25659 and CGS 24592) in human plasma by high-performance liquid chromatography.},
  author={W. Maniara and A. Cipriano and M. Powell},
  journal={Journal of chromatography. B, Biomedical sciences and applications},
  year={1998},
  volume={706 2},
  pages={
          287-94
        }
}
Two simple and sensitive reversed-phase high-performance liquid chromatography (HPLC) methods were developed and validated for the quantitative determination of a novel hypertension drug CGS 25462 and its major metabolites CGS 24592 and CGS 25659 in human plasma. CGS 25462 and CGS 25798 (internal standard) were purified by one-step liquid-liquid extraction with methylene chloride. The metabolites were analyzed on HPLC after plasma protein precipitation with 10% trichloroacetic acid (TCA… Expand
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References

Pharmacologic profile of CGS 24128, a potent, long-acting inhibitor of neutral endopeptidase 24.11.
TLDR
Thiorphan and CGS 24128 inhibited NEP in vitro with IC50 values of 5.0 +/-0.2 and 4.3 +/- 0.2 nM, respectively, and ANP-induced natriuresis was significantly potentiated in anesthetized rats pretreated with a bolus of CGS24128. Expand