Quantitative analysis of the relative transcript levels of ABC transporter Atr genes in Aspergillus nidulans by real-time reverse transcription-PCR assay.

@article{Semighini2002QuantitativeAO,
  title={Quantitative analysis of the relative transcript levels of ABC transporter Atr genes in Aspergillus nidulans by real-time reverse transcription-PCR assay.},
  author={Camile Pizeta Semighini and Mozart Marins and Maria Helena de Souza Goldman and Gustavo Henrique Goldman},
  journal={Applied and environmental microbiology},
  year={2002},
  volume={68 3},
  pages={1351-7}
}
The development of assays for quantitative analysis of the relative transcript levels of ABC transporter genes by real-time reverse transcription-PCR (RT-PCR) might provide important information about multidrug resistance in filamentous fungi. Here, we evaluate the potential of real-time RT-PCR to quantify the relative transcript levels of ABC transporter Atr genes from Aspergillus nidulans. The AtrA to AtrD genes showed different and higher levels in the presence of structurally unrelated… CONTINUE READING
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