Quantitative analysis of the immunosuppressant CP-690,550 in whole blood by column-switching high-performance liquid chromatography and mass spectrometry detection.

@article{Paniagua2005QuantitativeAO,
  title={Quantitative analysis of the immunosuppressant CP-690,550 in whole blood by column-switching high-performance liquid chromatography and mass spectrometry detection.},
  author={Ricardo T. Paniagua and Andrew D. Campbell and Paul S. Changelian and Bruce A. Reitz and Chandra Prakash and Dominic C. Borie},
  journal={Therapeutic drug monitoring},
  year={2005},
  volume={27 5},
  pages={
          608-16
        }
}
A fast and accurate method to quantify the new immunosuppressive JAK3 inhibitor CP-690,550 in whole blood using a dual-pump liquid chromatography-liquid chromatography-mass spectrometry (LC/LC-MS) system was developed and validated in nonhuman primate blood. Before injection, blood samples were prepared by precipitation with a reagent that included methanol and acetonitrile (30:70, vol/vol) along with the internal standard (CP-istd). Column-switching LC/LC-MS analysis used online extraction… CONTINUE READING