Quantitative analysis of the effect of cell type and cellular differentiation on protective antigen binding to human target cells.

@article{Deshpande2006QuantitativeAO,
  title={Quantitative analysis of the effect of cell type and cellular differentiation on protective antigen binding to human target cells.},
  author={Alina Deshpande and Rebecca J. Hammon and Claire K. Sanders and Steven W Graves},
  journal={FEBS letters},
  year={2006},
  volume={580 17},
  pages={4172-5}
}
We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined with the function of such cells, this suggests that PA receptors interact with the extracellular matrix and that differentiation increases the number of PA-specific receptors, which… CONTINUE READING