Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer.

@article{Beum2006QuantitativeAO,
  title={Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer.},
  author={Paul V. Beum and Margaret A Lindorfer and Brian Eric Hall and Thaddeus C. George and Keith L. Frost and Philip J. Morrissey and Ronald P Taylor},
  journal={Journal of immunological methods},
  year={2006},
  volume={317 1-2},
  pages={90-9}
}
Binding of the chimeric, humanized anti-CD20 mAb Rituximab (RTX) to B lymphocytes activates complement and promotes covalent deposition of C3 fragments (C3b/iC3b) on cells. Previous fluorescence microscopy studies, based on examination of B cell lines and of blood samples from RTX-treated CLL patients, suggest that C3b/iC3b is closely associated with cell-bound RTX. We examined Raji cells opsonized with serum and RTX with the ImageStream imaging flow cytometer. Cells were stained with… CONTINUE READING
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Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow

  • T. C. George, S. L. Fanning, +9 authors D. H. Lynch
  • J. Immunol. Methods
  • 2006
2 Excerpts

Efficacy and safety of Rituximab in active RA patients who experienced an inadequate response to one or more anti-TNFα therapies (REFLEX study)

  • S. B. Cohen, M. Greenwald, +4 authors M. C. Totoritis
  • Arthritis Rheum
  • 2005
1 Excerpt

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