Quantitative analysis of latent human cytomegalovirus.

  title={Quantitative analysis of latent human cytomegalovirus.},
  author={Barry Slobedman and Edward S. Mocarski},
  journal={Journal of virology},
  volume={73 6},
Cytomegalovirus latency depends on an interaction with hematopoietic cells in bone marrow and peripheral blood. The distribution of viral DNA was investigated by PCR-driven in situ hybridization (PCR-ISH), and the number of viral genomes per cell was estimated by quantitative competitive PCR during both experimental and natural latent infection. During experimental latent infection of cultured granulocyte-macrophage progenitors, the viral genome was detected in >90% of cells at a copy number of… CONTINUE READING