Quantitative analysis of in vitro ubiquitinated cyclin B1 reveals complex chain topology

Abstract

Protein ubiquitination regulates many cellular processes, including protein degradation, signal transduction, DNA repair and cell division. In the classical model, a uniform polyubiquitin chain that is linked through Lys 48 is required for recognition and degradation by the 26S proteasome. Here, we used a reconstituted system and quantitative mass… (More)
DOI: 10.1038/ncb1436

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