Quantitative analysis of human herpesvirus 8 viral load using a real-time PCR assay.

@article{Lallemand2000QuantitativeAO,
  title={Quantitative analysis of human herpesvirus 8 viral load using a real-time PCR assay.},
  author={Francis Lallemand and Nathalie D{\'e}sir{\'e} and Willy Rozenbaum and J. C. Nicolas and Val{\'e}rie Mar{\'e}chal},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 4},
  pages={1404-8}
}
We have developed a quantitative real-time PCR (TaqMan) assay aimed at measuring the cellular human herpesvirus 8 (HHV-8) DNA load in various clinical samples. Standard curves were obtained by serial dilutions of a control plasmid containing both HHV-8 (ORF73 gene) and the cellular target (human albumin gene). The assay appeared to be very sensitive (100% detection rate for at least 10 copies per well) and specific and was easily reproducible (less than 3% intra-assay variability, 5% interassay… CONTINUE READING
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