Quantitative analysis of human cerebrospinal fluid proteins using a combination of cysteine tagging and amine-reactive isobaric labeling.

@article{Giron2011QuantitativeAO,
  title={Quantitative analysis of human cerebrospinal fluid proteins using a combination of cysteine tagging and amine-reactive isobaric labeling.},
  author={Priscille Giron and Lo{\"i}c Dayon and Natacha Turck and Christine Hoogland and Jean-Charles Sanchez},
  journal={Journal of proteome research},
  year={2011},
  volume={10 1},
  pages={249-58}
}
Highly complex and dynamic protein mixtures are hardly comprehensively resolved by direct shotgun proteomic analysis. As many proteins of biological interest are of low abundance, numerous analytical methodologies have been developed to reduce sample complexity and go deeper into proteomes. The present work describes an analytical strategy to perform cysteinyl-peptide subset enrichment and relative quantification through successive cysteine and amine-isobaric tagging. A cysteine-reactive… CONTINUE READING

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