Quantitative analysis of herpesvirus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR.

@article{Quackenbush2001QuantitativeAO,
  title={Quantitative analysis of herpesvirus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR.},
  author={S. Quackenbush and R. N. Casey and R. Murcek and T. Paul and T. Work and C. Limpus and A. Chaves and L. duToit and J. V. P{\'e}rez and A. Aguirre and T. Spraker and J. Horrocks and L. Vermeer and G. Balazs and J. Casey},
  journal={Virology},
  year={2001},
  volume={287 1},
  pages={
          105-11
        }
}
Quantitative real-time PCR has been used to measure fibropapilloma-associated turtle herpesvirus (FPTHV) pol DNA loads in fibropapillomas, fibromas, and uninvolved tissues of green, loggerhead, and olive ridley turtles from Hawaii, Florida, Costa Rica, Australia, Mexico, and the West Indies. [...] Key Result The viral DNA loads from tumors obtained from terminal animals were relatively homogeneous (range 2-20 copies/cell), whereas DNA copy numbers from biopsied tumors and skin of otherwise healthy turtles…Expand
Molecular evolution of fibropapilloma-associated herpesviruses infecting juvenile green and loggerhead sea turtles.
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A database containing DNA from over 400 tissue samples taken from green and loggerhead marine turtles, including multiple tissue types, a twenty year time span, and both diseased and asymptomatic animals, finds that nested PCR across multiple loci out-performed qPCR and is a more powerful technique for determining infection status. Expand
Localization of fibropapilloma-associated turtle herpesvirus in green turtles (Chelonia mydas) by in-situ hybridization.
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Results suggest that FPTHV is present in epithelial cells and transcriptionally active in fibropapillomas, and may be present within the tumours of green turtles examined in Puerto Rico. Expand
Genomic Variation of the Fibropapilloma-Associated Marine Turtle Herpesvirus across Seven Geographic Areas and Three Host Species
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The results indicate that FPTHV was established in marine turtle populations prior to the emergence of FP as it is currently known, and is a member of the Alphaherpesvirinae. Expand
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High viral loads correlated positively with increasing FP severity in turtles sampled on the Brazilian coast and with samples from turtles found dead in the states of São Paulo and Bahia. Expand
Detection and characterization of fibropapilloma associated herpesvirus of marine turtles in Rio Grande do Sul, Brazil
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The first molecular characterization of herpesvirus associated with fibropapilloma in turtles from the coast of RS is reported, with results that suggest the involvement of a herpesv virus in association with environmental and genetic factors as causal agents of FP. Expand
Presence of chelonid fibropapilloma-associated herpesvirus in tumored and non-tumored green turtles, as detected by polymerase chain reaction, in endemic and non-endemic aggregations, Puerto Rico
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Identification of CFPHV harbored in a non-epidermal site (dermis) of a tumor-free turtle indicates that virus is latent in aNon-tumored host, supporting the idea that extrinsic and/or host factors could play a transforming role in tumor expression. Expand
The Ozobranchus leech is a candidate mechanical vector for the fibropapilloma-associated turtle herpesvirus found latently infecting skin tumors on Hawaiian green turtles (Chelonia mydas).
TLDR
Quantitative RT-PCR analysis ofFPTHV gene expression indicated that most of the FPTHV copies in a fibropapilloma have restricted DNA polymerase expression, suggestive of latent infection. Expand
Further evidence of Chelonid herpesvirus 5 (ChHV5) latency: high levels of ChHV5 DNA detected in clinically healthy marine turtles
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The theory that ChHV5 is a near ubiquitous virus with latency characteristics requiring co-factors, possibly environmental or immune related, to induce FP is supported. Expand
Chelonid herpesvirus 5 in secretions and tumor tissues from green turtles (Chelonia mydas) from Southeastern Brazil: A ten-year study.
TLDR
Phylogenetic analysis indicated that Brazilian samples were similar to ChHV5 samples described for the Atlantic phylogeographic group and are therefore part of the same clade as the Gulf of Guinea and Puerto Rico samples, which suggests a possible flow of the virus between these three regions. Expand
Examining the Role of Transmission of Chelonid Alphaherpesvirus 5
TLDR
The presence of ChHV5 DNA in swabs suggests a possible route of viral transmission through viral secretion and excretion via corporal fluids in turtles without FP tumors, which was detected more readily in skin biopsies than swabs. Expand
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Three closely related herpesviruses are associated with fibropapillomatosis in marine turtles.
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The data suggest that these closely related turtle herpesviruses are intimately involved in the genesis of fibropapillomatosis. Expand
Association of herpesvirus with fibropapillomatosis of the green turtle Chelonia mydas and the loggerhead turtle Caretta caretta in Florida.
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Results lead to the conclusion that a chelonian herpesvirus is regularly associated with fibropapillomatosis and is not merely an incidental finding in affected turtles. Expand
Cutaneous fibropapillomas of green turtles (Chelonia mydas).
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Six juvenile green turtles from the Indian River Lagoon System, Florida, U.S.A., with multiple cutaneous fibropapillomas, were kept in isolation and examined over a 6-month period, demonstrating membrane-bound intracytoplasmic vacuoles within epidermal cells in the stratum basale and within the dermis. Expand
The potential role of natural tumor promoters in marine turtle fibropapillomatosis
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It is shown that green turtles in the Hawaiian Islands consume Prorocentrum and that high-risk FP areas are associated with areas where P. lima and P. concavum are both highly prevalent and abundant. Expand
Comparative Pathology and Pathogenesis of Spontaneous and Experimentally Induced Fibropapillomas of Green Turtles (Chelonia mydas)
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The possible involvement of GTFP-associated herpesvirus in the pathogenesis of epidermal degenerative changes and GTFP pathogenesis is discussed. Expand
Sensitivity of the transmissible green turtle fibropapillomatosis agent to chloroform and ultracentrifugation conditions
TLDR
Tumorigenic activity survived storage for several months at -80°C and at least 1 yr at -180°C, making it feasible to isolate the GTFP agent and its genome from frozen archived material once methods have been optirnized. Expand
Herpesvirus in cutaneous fibropapillomas of the green turtle Chelonia mydas
TLDR
Two juvenile green turtles Chelonia rnydas with multiple cutaneous and ocular fibropapillomas were evaluated and areas of ballooning degeneration of epidermal cells associated with eosinophilic intranuclear inclusions found were compatible with those of the family Herpetoviridae. Expand
Detection and analysis of diverse herpesviral species by consensus primer PCR
TLDR
Estimation of template dilution studies in the presence of human carrier DNA demonstrated that six human herpesviruses could be detected at levels at or below 100 genome equivalents per 100 ng of carrier DNA, suggesting that consensus primer PCR targeted to herpesviral DNA polymerase may prove to be useful in the detection and identification of known herpesvirus in clinical samples and the initial characterization of new herpesViral genomes. Expand
Green turtle fibropapillomatosis: challenges to assessing the role of environmental cofactors.
TLDR
Alternative explanations for high GTFP prevalence in some near-shore habitats include the following: a) these habitats provide an optimum physical environment for survival and transmission of the infectious agent, and these habitats attract a high density of susceptible turtles or harbor a higher density of potential vectors, facilitating Transmission of the pathogen in a density-dependent fashion. Expand
Cutaneous Fibropapillomas and Renal Myxofibroma in a Green Turtle, Chelonia mydas
A debilitated 7 kg juvenile green turtle (Chelonia mydas mydas) with multiple ulcerated and infected cutaneous fibropapillomas was clinically evaluated and found to have a nonregenerative anemia,Expand
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