Quantitative analysis of herpesvirus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR.

  title={Quantitative analysis of herpesvirus sequences from normal tissue and fibropapillomas of marine turtles with real-time PCR.},
  author={Sandra L. Quackenbush and Rufina N. Casey and Roman Murcek and Thomas A Paul and Thierry M. Work and Colin James Limpus and Anny Chaves and Lindsey duToit and J. Valdimir P{\'e}rez and A. Alonso Aguirre and T. R. Spraker and Julia A. Horrocks and L. A. Vermeer and George H. Balazs and James W. Casey},
  volume={287 1},
Quantitative real-time PCR has been used to measure fibropapilloma-associated turtle herpesvirus (FPTHV) pol DNA loads in fibropapillomas, fibromas, and uninvolved tissues of green, loggerhead, and olive ridley turtles from Hawaii, Florida, Costa Rica, Australia, Mexico, and the West Indies. [] Key Result The viral DNA loads from tumors obtained from terminal animals were relatively homogeneous (range 2-20 copies/cell), whereas DNA copy numbers from biopsied tumors and skin of otherwise healthy turtles…

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