Quantitative analysis of gene-specific DNA damage in human spermatozoa.

@article{Sawyer2003QuantitativeAO,
  title={Quantitative analysis of gene-specific DNA damage in human spermatozoa.},
  author={Dennis E. Sawyer and Belinda G Mercer and Agnieszka M Wiklendt and Robert John Aitken},
  journal={Mutation research},
  year={2003},
  volume={529 1-2},
  pages={
          21-34
        }
}
Recent studies have suggested that human spermatozoa are highly susceptible to DNA damage induced by oxidative stress. However, a detailed analysis of the precise nature of this damage and the extent to which it affects the mitochondrial and nuclear genomes has not been reported. To induce DNA damage, human spermatozoa were treated in vitro with hydrogen peroxide (H2O2; 0-5 mM) or iron (as Fe(II)SO4, 0-500 microM). Quantitative PCR (QPCR) was used to measure DNA damage in individual nuclear… CONTINUE READING

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