Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture.

@article{Marlovits2004QuantitativeAO,
  title={Quantitative analysis of gene expression in human articular chondrocytes in monolayer culture.},
  author={Stefan Marlovits and Matthias Hombauer and Dietmar Tamandl and Vilmos V{\'e}csei and Werner Schlegel},
  journal={International journal of molecular medicine},
  year={2004},
  volume={13 2},
  pages={281-7}
}
Human articular chondrocytes (HACs) were isolated from cartilage samples of normal joints and cultivated in monolayer for 56 days. Collagen type I, II, IX, aggrecan, and versican expression was quantified by real-time polymerase chain reaction (PCR). The expression of the genes was highly time-dependent and changed over the culture time. Collagen type I was not present at the beginning of the culture and increased 100-fold during the culture time. Collagen type II and IX expression was found… CONTINUE READING