Quantitative analysis of energy transfer between fluorescent proteins in CFP – GBP – YFP and its response to Ca 2 + w

Abstract

This article reports the full characterisation of the optical properties of a biosynthesised protein consisting of fused cyan fluorescent protein, glucose binding protein and yellow fluorescent protein. The cyan and yellow fluorescent proteins act as donors and acceptors for intramolecular fluorescence resonance energy transfer. Absorption, fluorescence… (More)

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