Quantitative analysis of despropionyl-bezitramide, the active metabolite of bezitramide (Burgodin), in biological samples by high-performance liquid chromatography with fluorescence detection.

@article{DeBaere1997QuantitativeAO,
  title={Quantitative analysis of despropionyl-bezitramide, the active metabolite of bezitramide (Burgodin), in biological samples by high-performance liquid chromatography with fluorescence detection.},
  author={Siegrid De Baere and Willy E E Lambert and A P De leenheer},
  journal={Analytical chemistry},
  year={1997},
  volume={69 24},
  pages={
          5186-92
        }
}
A sensitive high-performance liquid chromatographic procedure with fluorescence detection was developed for the quantitative determination of despropionyl-bezitramide, the active metabolite of bezitramide (Burgodin), in biological samples. Chromatographic separation was achieved on a Hypersil ODS (C18) 5-micron column, using a 31:69 (v/v) mixture of 0.1 M ammonium acetate and methanol/acetonitrile (50:50, v/v)-0.1 M ammonium acetate as the eluent. Internal standardization with N…