Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience

Abstract

Short tandem repeat (STR) markers are increasingly used for monitoring the engraftment of donor cells after stem cell transplantation.1–8 In this setting, donor:recipient chimerism in highly purified leukocyte subsets seems to be more informative for detection of graft failure or relapse of the disease than quantification of chimerism in whole peripheral blood (PB) or bone marrow (BM).1,3,4,8–11 Determination of donor chimerism should lead to comparable results in different laboratories, which implies that results ought to be independent of the STR primers and the detection system used. We compared a singleplex in-house STR primer set PCR (Table 1) with two commercially available multiplex PCR systems (AmpFISTER Profiler,5–7,13,14 AmpFISTER SGM Plus PCR amplification kit;7,15 Applied Biosystems/ABI/, Weiterstadt, Germany) in a patient with thalassemia. PCR products were assessed by both capillary electrophoresis (ABI Prism 310 genetic analyzer) and polyacrylamide gel electrophoresis (PAGE) systems (ABI Prism 373 DNA sequencer).

DOI: 10.1038/sj.leu.2402760

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@article{Koehl2003QuantitativeAO, title={Quantitative analysis of chimerism after allogeneic stem cell transplantation by PCR amplification of microsatellite markers and capillary electrophoresis with fluorescence detection: the Frankfurt experience}, author={Ulrike Koehl and Otfried Beck and Ronald Esser and Erhard Seifried and T E Klingebiel and Dirk Schwabe and Christian Seidl}, journal={Leukemia}, year={2003}, volume={17}, pages={232-236} }