Quantitative analysis of cell cycle phase durations and PC12 differentiation using fluorescent biosensors.

@article{Hahn2009QuantitativeAO,
  title={Quantitative analysis of cell cycle phase durations and PC12 differentiation using fluorescent biosensors.},
  author={Angela T. Hahn and Joshua T. Jones and Tobias Meyer},
  journal={Cell cycle},
  year={2009},
  volume={8 7},
  pages={1044-52}
}
Cell cycle analysis typically relies on fixed time-point measurements of cells in particular phases of the cell cycle. The cell cycle, however, is a dynamic process whose subtle shifts are lost by fixed time-point methods. Live-cell fluorescent biosensors and time-lapse microscopy allows the collection of temporal information about real time cell cycle progression and arrest. Using two genetically-encoded biosensors, we measured the precision of the G(1), S, G(2) and M cell cycle phase… CONTINUE READING

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