Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction.

@article{Lo1999QuantitativeAO,
  title={Quantitative analysis of aberrant p16 methylation using real-time quantitative methylation-specific polymerase chain reaction.},
  author={Y. M. Dennis Lo and Ivy Hoi-Ka Wong and Jing Zhang and M S Tein and Mun Hon Ng and N. Magnus Hjelm},
  journal={Cancer research},
  year={1999},
  volume={59 16},
  pages={3899-903}
}
We have developed a quantitative method for methylation analysis of the p16 gene based on real-time methylation-specific PCR (MSP). Real-time MSP is sensitive enough to detect down to 10 genome equivalents of the methylated p16 sequence. Application of real-time MSP to DNA from tumor-derived cell lines revealed complete concordance with conventional MSP analysis. Quantitative data generated by real-time MSP were expressed as the methylation index, which was defined as the percentage of… CONTINUE READING
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