Quantitative analysis of F‐actin redistribution in astrocytoma cells treated with candidate pharmaceuticals

  title={Quantitative analysis of F‐actin redistribution in astrocytoma cells treated with candidate pharmaceuticals},
  author={Stephen J. Lockett and Chrissie Verma and Alla Brafman and Prabhakar R. Gudla and Kaustav Nandy and Yoshihiro Mimaki and Philip L. Fuchs and Joseph Jaja and Karlyne M. Reilly and John A. Beutler and Thomas Turbyville},
  journal={Cytometry Part A},
Actin fibers (F‐actin) control the shape and internal organization of cells, and generate force. It has been long appreciated that these functions are tightly coupled, and in some cases drive cell behavior and cell fate. The distribution and dynamics of F‐actin is different in cancer versus normal cells and in response to small molecules, including actin‐targeting natural products and anticancer drugs. Therefore, quantifying actin structural changes from high resolution fluorescence micrographs… Expand
A spatiotemporal characterization method for the dynamic cytoskeleton
This method differentiates between normal and chemically disrupted actin networks, and quantifies rates of cytoskeletal degradation, and can be further implemented to gain insights on dynamic growth, disruption, and structure of the cytoskeleton (and other complex biological morphology) due to biological, chemical, or physical stimuli. Expand
MACC1 driven alterations in cellular biomechanics facilitate cell motility in glioblastoma
Targeting re-organization of the cytoskeleton and cell motility via MACC1 may offer a treatment option to impede GBM spreading. Expand
Unbiased pattern analysis reveals highly diverse responses of cytoskeletal systems to cyclic straining
It was found that cyclic stretching affected the actin cytoskeleton most, microtubules less, and vimentin mostly only via reorientation of the whole cell, which decoupled the cytoskeletal systems as it reduced the cross-correlations in both cases. Expand
Anti-tumor activity of chlorogenic acid by regulating the mTORC 2 signaling pathway and disrupting F-actin organization
Chlorogenic acid (CGA), a plant-based dietary polyphenol exhibits potential anti-cancer capability. However, the precise molecular mechanisms remain to be elucidated. In the present study, theExpand
Rapha Myr®, a Blend of Sulforaphane and Myrosinase, Exerts Antitumor and Anoikis-Sensitizing Effects on Human Astrocytoma Cells Modulating Sirtuins and DNA Methylation
Rapha Myr® exhibited low antioxidant capability and exerted antiproliferative and genotoxic effects on 1321N1 cells by blocking the cell cycle, disarranging cytoskeleton structure and focal adhesions, decreasing the integrin α5 expression, renewing anoikis and modulating some important epigenetic pathways independently of the cellular p53 status. Expand
Schweinfurthins: Lipid Modulators with Promising Anticancer Activity.
A in-depth look at the history of schweinfurthins, their synthesis, where the research presently stands, and the questions that remain is taken. Expand
Calcium and P-glycoprotein independent synergism between schweinfurthins and verapamil
These studies support the hypothesis that verapamil potentiates the schweinfurthin growth inhibitory effect by increasing its intracellular concentration, and co-treatment with cholesterol rescued the methyl-G-induced increase in Rhodamine 123 intrACEllular concentration. Expand


A quantitative measure for alterations in the actin cytoskeleton investigated with automated high‐throughput microscopy
This work benchmarked its analysis by calculating coherency for both a biophysical model of the actin cytoskeleton and for cells whose actin architecture had been disturbed pharmacologically by latrunculin B or cytochalasin D, and observed no significant differences between uninfected and infected cells. Expand
Electron tomography reveals unbranched networks of actin filaments in lamellipodia
In lamellipodia from four cell types, including rapidly migrating fish keratocytes, it is found that actin filaments are almost exclusively unbranched, providing a new structural basis for understanding actin-driven protrusion during cell migration. Expand
Fractal and Image Analysis of Morphological Changes in the Actin Cytoskeleton of Neonatal Cardiac Fibroblasts in Response to Mechanical Stretch
Image analysis and non-Euclidian geometrical fractal analysis are applied to quantify and describe changes induced in the actin cytoskeleton of cardiac fibroblasts responding to mechanical stress to provide insight into the regulation of fibro Blasts behavior and gene expression during heart development and disease. Expand
Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.
How motile cells regulate actin filament assembly at their leading edge is reviewed, including how Arp2/3 complex is incorporated into the network, and new filaments are capped rapidly, so that activated Arp1/2 complex must be supplied continuously to keep the network growing. Expand
Analysis of actin filament network organization in lamellipodia by comparing experimental and simulated images
This work performed simulations of actin network assembly, based on the hypothesis of stochastic branching and capping of filaments, and produced computed `fluorescence' and `electron microscopy' images of the simulated network, and closely reproduced the contrast and the characteristic diagonal criss-cross pattern observed in the experimental optical images. Expand
Actin, a Central Player in Cell Shape and Movement
Comparisons of quantitative measurements of reactions in live cells with computer simulations of mathematical models will help generate meaningful insights and present a summary of the key questions in the field. Expand
Multiple spatiotemporal modes of actin reorganization by NMDA receptors and voltage-gated Ca2+ channels
It is suggested that spatiotemporal dynamics of [Ca2+]i, triggered by activation of N-methyl-d-aspartate receptors and voltage-gated Ca2+ channels, provides the molecular basis for activity-dependent actin remodeling. Expand
Anisotropy of cell adhesive microenvironment governs cell internal organization and orientation of polarity
It is demonstrated that ECM geometry determines the orientation of cell polarity axes and proposed a working model that accounts for that observation concludes that, in addition to molecular composition and mechanical properties,ECM geometry plays a key role in developmental processes. Expand
The many faces of actin: matching assembly factors with cellular structures
This work focuses on structures at the plasma membrane, including both sheet-like protrusive structures and finger-like protrusions (such as filopodia and microvilli) and insights gained from studies of adherens junctions and the immunological synapse are considered. Expand
Extraction and analysis of actin networks based on Open Active Contour models
A fully automated method to extract actin networks from images and analyze network topology is proposed, which handles well intersecting filaments and, to some extent, overlapping filaments. Expand