Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter.

@article{Kamiya2010QuantitativeYS,
  title={Quantitative Y2H screening: cloning and signal peptide engineering of a fungal secretory LacA gene and its application to yeast two-hybrid system as a quantitative reporter.},
  author={Takuma Kamiya and Teruyo Ojima and Kanoko Sugimoto and Hideo Nakano and Yasuaki Kawarasaki},
  journal={Journal of biotechnology},
  year={2010},
  volume={146 4},
  pages={
          151-9
        }
}
A quantitative protein/peptide screening system amenable to high-throughput screening has been developed by furnishing conventional yeast two-hybrid (Y2H) system with an engineered fungal secretory beta-galactosidase gene (designated LacA3). We describe the molecular cloning and signal peptide-optimization of the original fungal LacA gene of which extracellular expression was initially toxic to the host cell. The engineered LacA, LacA3, showed less toxicity, resulting in improved cultural… CONTINUE READING

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