Quantitative Secretome Analysis of Activated Jurkat Cells Using Click Chemistry-Based Enrichment of Secreted Glycoproteins.

@article{Witzke2017QuantitativeSA,
  title={Quantitative Secretome Analysis of Activated Jurkat Cells Using Click Chemistry-Based Enrichment of Secreted Glycoproteins.},
  author={Kathrin E Witzke and Kristin Rosowski and Christian M{\"u}ller and Maike Ahrens and Martin Eisenacher and Dominik A Megger and J{\"u}rgen Knobloch and Andrea U. Koch and Thilo Bracht and Barbara Sitek},
  journal={Journal of proteome research},
  year={2017},
  volume={16 1},
  pages={
          137-146
        }
}
Quantitative secretome analyses are a high-performance tool for the discovery of physiological and pathophysiological changes in cellular processes. However, serum supplements in cell culture media limit secretome analyses, but serum depletion often leads to cell starvation and consequently biased results. To overcome these limiting factors, we investigated a model of T cell activation (Jurkat cells) and performed an approach for the selective enrichment of secreted proteins from conditioned… CONTINUE READING
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