Quantitative Rodent Brain Receptor Imaging

  title={Quantitative Rodent Brain Receptor Imaging},
  author={Kristina Herfert and Julia G. Mannheim and Laura Kuebler and Sabina Marciano and Mario Amend and Ch. Parl and Hanna Napieczynska and Florian M. Maier and Salvador Castaneda Vega and Bernd J. Pichler},
  journal={Molecular Imaging and Biology},
Positron emission tomography (PET) is a non-invasive imaging technology employed to describe metabolic, physiological, and biochemical processes in vivo . These include receptor availability, metabolic changes, neurotransmitter release, and alterations of gene expression in the brain. Since the introduction of dedicated small-animal PET systems along with the development of many novel PET imaging probes, the number of PET studies using rats and mice in basic biomedical research tremendously… 
Role of Nuclear Imaging to Understand the Neural Substrates of Brain Disorders in Laboratory Animals: Current Status and Future Prospects
The role of SPECT and PET small animal imaging systems for a better understanding of brain functioning in health and disease states is addressed and the changes in metabolic and neurotransmitter activity in various brain areas during task-induced neural activation are examined.
Recent Technical Advances in Accelerating the Clinical Translation of Small Animal Brain Imaging: Hybrid Imaging, Deep Learning, and Transcriptomics
The recent developments in small animal neuroimaging toward improved translational power are summarized, with a focus on technical improvement including hybrid imaging, data processing, transcriptomics, awake animal imaging, and on-chip pharmacokinetics.
Imaging Kappa Opioid Receptors in the Living Brain with Positron Emission Tomography.
The potential of KOR PET to detect changes in endogenous dynorphin in the human brain, to elucidate neural mechanisms and offer critical insight into disease-modifying therapeutics is discussed, and comments on other translational neuroimaging modalities such as MRI that could be used to study KOR-dynorphin tone in the living human brain are concluded.
Awake 18F-FDG PET Imaging of Memantine-Induced Brain Activation and Test–Retest in Freely Running Mice
The present method enables brain PET imaging on awake mice, thereby avoiding the confounding effects of anesthesia on the PET reading, and it allows the simultaneous measurement of behavioral information during PET acquisitions.
Compensating Positron Range Effects of Ga-68 in Preclinical PET Imaging by Using Convolutional Neural Network: A Monte Carlo Simulation Study
CNN3 should be a good candidate architecture for positron range correction in Ga-68 preclinical PET imaging and outperformed CNN1 and CNN2 qualitatively and quantitatively, according to the results.
Multimodal Contrast Agents for Optoacoustic Brain Imaging in Small Animals
Optoacoustic (photoacoustic) imaging has demonstrated versatile applications in biomedical research, visualizing the disease pathophysiology and monitoring the treatment effect in an animal model, as
Correlated Multimodal Imaging in Life Sciences: Expanding the Biomedical Horizon
A comprehensive overview of the field of CMI from preclinical hybrid imaging to correlative microscopy is presented, requirements for optimization and standardization are highlighted, and current efforts to bridge the gap between preclinical and biological imaging are focused on.
Characterization of an APP/tau rat model of Alzheimer’s disease by positron emission tomography and immunofluorescent labeling
Although relatively low human tau and amyloid fibril expression levels and a somewhat unstable brain pathology are observed, this model is thoroughly characterized using combined PET imaging and immunofluorescence analysis, which questions the utility of this animal model for further studies.
Imaging SERT Availability in a Rat Model of L-DOPA-Induced Dyskinesia
The authors' longitudinal PET data support a preservation of SERT availability after the induction of L-DOPA-induced dyskinesia, which is in line with previous reports in dyskinetic PD patients.
A novel antioxidant ergothioneine PET radioligand for in vivo imaging applications
This is the first time the biodistribution of this antioxidant and rare amino acid has been demonstrated in a preclinical mouse model in a highly sensitive and non-invasive manner.


Small animal PET imaging.
The basic principles of PET instrumentation and radiopharmaceuticals are discussed, the key factors responsible for the qualitative and quantitative imaging capabilities of small animal PET systems are examined, and the criteria that PET imaging agents must meet are described.
Fundamentals of Positron Emission Tomography and Applications in Preclinical Drug Development
  • S. Cherry
  • Biology
    Journal of clinical pharmacology
  • 2001
The fundamental basis of PET imaging is reviewed and the development of small‐animal PET scanners and their possible application in preclinical drug development are discussed.
Preclinical Experimentation in Neurology
This chapter will give a detailed description of each method and provide practical protocols to ensure that the data can be collected, analyzed, and interpreted in an unbiased manner.
Preclinical and Translational PET/MR Imaging
This article reviews studies in preclinical and clinical translation that provide additional insights into biologic systems that were not clearly feasible with just one modality alone and calls for more multidisciplinarity in the field of molecular imaging.
Noninvasive Nuclear Imaging Enables the In Vivo Quantification of Striatal Dopamine Receptor Expression and Raclopride Affinity in Mice
The data showed that the tracer mass, if higher than 4 nmol/kg, can strongly affect binding parameter estimations and must be considered when performing kinetic analysis, specifically in mice.
Simultaneous PET-MRI: a new approach for functional and morphological imaging
This paper introduces a new technology synergizing two leading imaging methodologies: positron emission tomography (PET) and magnetic resonance imaging (MRI), and develops a three-dimensional animal PET scanner that is built into a 7-T MRI.
Simultaneous in vivo positron emission tomography and magnetic resonance imaging
In vivo simultaneous PET and MRI studies were performed in mice and the signal-to-noise ratio and uniformity of the MR images, with the exception of one particular pulse sequence, were little affected by the presence of the PET scanner.
Imaging Synaptic Neurotransmission with in Vivo Binding Competition Techniques: A Critical Review
  • M. Laruelle
  • Biology
    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism
  • 2000
The results suggest that the changes in BP following changes in DA concentration might not be fully accounted by a simple occupancy model, and the data are reviewed supporting that agonist-mediated receptor internalization might play an important role in characterizing receptor-ligand interactions.