Quantitative Real-Time PCR Detection of Toxic Nodularia Cyanobacteria in the Baltic Sea

@article{Koskenniemi2007QuantitativeRP,
  title={Quantitative Real-Time PCR Detection of Toxic Nodularia Cyanobacteria in the Baltic Sea},
  author={Kerttu Koskenniemi and Christina Lyra and Pirjo Rajaniemi-Wacklin and Jouni Kalevi Jokela and Kaarina Sivonen},
  journal={Applied and Environmental Microbiology},
  year={2007},
  volume={73},
  pages={2173 - 2179}
}
ABSTRACT A specific quantitative real-time PCR (qPCR) method was developed for the quantification of hepatotoxin nodularin-producing Nodularia, one of the main bloom-forming cyanobacteria in the Baltic Sea. Specific PCR primers were designed for subunit F of the nodularin synthetase gene (ndaF), which encodes the NdaF subunit of the nodularin synthetase gene complex needed for nodularin production. The qPCR method was applied to water samples (a total of 120 samples) collected from the Baltic… Expand
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