Quantitative RT-PCR: pitfalls and potential.

@article{Freeman1999QuantitativeRP,
  title={Quantitative RT-PCR: pitfalls and potential.},
  author={Willard M Freeman and Sarah J. Walker and Kent E. Vrana},
  journal={BioTechniques},
  year={1999},
  volume={26 1},
  pages={
          112-22, 124-5
        }
}
Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing RNA. While it has tremendous potential for quantitative applications, a comprehensive knowledge of its technical aspects is required. Successful quantitative RT-PCR involves correction for experimental variations in individual RT and PCR efficiencies. This review addresses the mathematics of RT-PCR, choice of RNA standards (internal vs. external) and quantification strategies (competitive, noncompetitive… CONTINUE READING
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