Quantitative RT-PCR: pitfalls and potential.

  title={Quantitative RT-PCR: pitfalls and potential.},
  author={Willard M Freeman and Sarah J. Walker and Kent E. Vrana},
  volume={26 1},
          112-22, 124-5
Reverse transcription PCR (RT-PCR) represents a sensitive and powerful tool for analyzing RNA. While it has tremendous potential for quantitative applications, a comprehensive knowledge of its technical aspects is required. Successful quantitative RT-PCR involves correction for experimental variations in individual RT and PCR efficiencies. This review addresses the mathematics of RT-PCR, choice of RNA standards (internal vs. external) and quantification strategies (competitive, noncompetitive… CONTINUE READING
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Publications referenced by this paper.
Showing 1-10 of 44 references

[Real time quantitative PCR].

Experimental & molecular medicine • 2001

Key issues , challenges , and future opportunities in gene quantification , p . 1 - 16

F. Ferré, A. Marchese, S. Grif-fin, E. Buxton, V. Boyer

Quantification of specific nucleic acids , regulated RNA processing , and genomic polymorphisms using reverse - phase HPLC , p . 45 - 78

F. Ferré, A. Hayward-Lester

Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity

T. Giles, A. Tucker
BioTechniques • 1997

A novel method for real time quantitative RT - PCR

P. M. Williams
Genome Res . • 1996

Evalua - tion of electrochemiluminescence - and bioluminescence - based assays for quantitating specific DNA

W. Rostène, P. Forgez
J . Clin . Lab . Anal . • 1996

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