Quantitative PET Reporter Gene Imaging with [11C]Trimethoprim.

  title={Quantitative PET Reporter Gene Imaging with [11C]Trimethoprim.},
  author={Mark A Sellmyer and Iljung Lee and Catherine Hou and Brian P. Lieberman and Chenbo Zeng and David A. Mankoff and Robert H. Mach},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  volume={25 1},
There is a need for improved methods to image genetically engineered cells, including immune cells used for cell-based therapy. Given the genetic manipulation inherent to gene therapy, the use of a reporter protein is a logical solution and positron emission tomography (PET) can provide the desired sensitivity and spatial localization. We developed a broadly applicable PET imaging strategy based on the small bacterial protein E. coli dihydrofolate reductase (Ec dhfr) and its highly specific… CONTINUE READING