Quantitative PCR analysis for fruit juice authentication using PCR and laboratory-on-a-chip capillary electrophoresis according to the Hardy-Weinberg law.

@article{Scott2009QuantitativePA,
  title={Quantitative PCR analysis for fruit juice authentication using PCR and laboratory-on-a-chip capillary electrophoresis according to the Hardy-Weinberg law.},
  author={Mary Scott and Angus Knight},
  journal={Journal of agricultural and food chemistry},
  year={2009},
  volume={57 11},
  pages={
          4545-51
        }
}
DNA-based analysis for the authentication of fruit juices was evaluated using the Polymerase Chain Reaction (PCR) and laboratory-on-a-chip capillary electrophoresis (LOC). A PCR restriction fragment length polymorphism (RFLP) assay demonstrated the detection of grapefruit juice in orange juice, although the assay was relatively insensitive with a limit of detection of 10% v/v. A PCR heteroduplex assay for detecting mandarin juice in orange juice was successfully applied to the LOC system and… CONTINUE READING