Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes.

@article{Selenko2006QuantitativeNA,
  title={Quantitative NMR analysis of the protein G B1 domain in Xenopus laevis egg extracts and intact oocytes.},
  author={Philipp Selenko and Zach Serber and Bedrick B Gadea and Joan V. Ruderman and Gerhard Wagner},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2006},
  volume={103 32},
  pages={11904-9}
}
We introduce a eukaryotic cellular system, the Xenopus laevis oocyte, for in-cell NMR analyses of biomolecules at high resolution and delineate the experimental reference conditions for successful implementations of in vivo NMR measurements in this cell type. This approach enables quantitative NMR experiments at defined intracellular concentrations of exogenous proteins, which is exemplified by the description of in-cell NMR properties of the protein G B1 domain (GB1). Additional experiments in… CONTINUE READING

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