Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-Based Probes.

@article{Lismont2017QuantitativeMO,
  title={Quantitative Monitoring of Subcellular Redox Dynamics in Living Mammalian Cells Using RoGFP2-Based Probes.},
  author={Celien Lismont and P. Walton and M. Fransen},
  journal={Methods in molecular biology},
  year={2017},
  volume={1595},
  pages={
          151-164
        }
}
To gain additional insight into how specific cell organelles may participate in redox signaling, it is essential to have access to tools and methodologies that are suitable to monitor spatiotemporal differences in the levels of different reactive oxygen species (ROS) and the oxidation state of specific redox couples. Over the years, the use of genetically encoded fluorescent redox indicators with a ratiometric readout has constantly gained in popularity because they can easily be targeted to… Expand
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  • M. Fransen
  • Biology, Medicine
  • Methods in molecular biology
  • 2014
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