Quantitative Monitoring of Solid-Phase Synthesis Using Gated Decoupling 13 C NMR Spectroscopy with a 13 C-Enriched Protecting Group and an Internal Standard in the Synthesis of Sialyl LewisX Tetrasaccharide.

@article{Kanemitsu1998QuantitativeMO,
  title={Quantitative Monitoring of Solid-Phase Synthesis Using Gated Decoupling 13 C NMR Spectroscopy with a 13 C-Enriched Protecting Group and an Internal Standard in the Synthesis of Sialyl LewisX Tetrasaccharide.},
  author={Takuya Kanemitsu and Osamu Kanie and Chi-Huey Wong},
  journal={Angewandte Chemie},
  year={1998},
  volume={37 24},
  pages={
          3415-3418
        }
}
Just tagging along. For the nondestructive quantitative monitoring of solid-phase oligosaccharide synthesis a 13 C-enriched tag (*) was incorporated in the linker and a 13 C-enriched protecting group (⧫) was included in the growing molecule. By integration of the signals in a gated decoupling 13 C NMR experiment the reaction progress can be monitored. This method was demonstrated with the synthesis of sialyl Lewisx tetrasaccharide on a Tentagel support ((P)). Bn=benzyl, PEG=poly(ethylene glycol… CONTINUE READING

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