Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

@article{Schena1995QuantitativeMO,
  title={Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray},
  author={Mark Schena and Dari Shalon and Ronald W. Davis and Patrick O. Brown},
  journal={Science},
  year={1995},
  volume={270},
  pages={467 - 470}
}
A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential… 
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References

SHOWING 1-10 OF 20 REFERENCES

Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones

The more than 13,400 plant ESTs that are currently available provide a new resource that will facilitate progress in many areas of plant biology and are illustrated by a description of the results obtained from analysis of 1500 Arabidopsis ESTs from a cDNA library prepared from equal portions of poly(A+) mRNA from etiolated seedlings, roots, leaves, and flowering inflorescence.

Characterization of the genome of Arabidopsis thaliana.

An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana.

As part of the goal to generate a detailed transcript map for Arabidopsis thaliana, 1152 single run sequences (expressed sequence tags or ESTs) have been determined from cDNA clones taken at random…

Identification and map position of YAC clones comprising one-third of the Arabidopsis genome.

The results presented here represent a first step towards assembly of an overlapping YAC library of the A. thaliana genome.

Assessing mapping progress in the Human Genome Project.

Four measures that should be applied to any mapping project to describe its progress are outlined, believing that universal measures of mapping progress are both desirable and possible.

Arabidopsis thaliana and Plant Molecular Genetics

Arabidopsis thaliana is a small flowering plant with various properties that make it an excellent organism for experiments in molecular genetics. These properties include having a small nuclear…

The human genome project. Prospects and implications for clinical medicine.

The recently initiated human genome project is a large international effort to elucidate the genetic architecture of the genomes of man and several model organisms and will have a profound impact on clinical medicine.

HD-Zip proteins: members of an Arabidopsis homeodomain protein superfamily.

  • M. SchenaR. W. Davis
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1992
It is concluded that Arabidopsis encodes a large family of homeodomain proteins, including members that contain a homeodOMain/leucine-zipper (HD-Zip) motif.

The HAT4 gene of Arabidopsis encodes a developmental regulator.

The HAT4 gene of Arabidopsis encodes an HD-Zip protein that functions as a novel developmental regulator that corresponds to highly conserved positions in known homeo domains and leucine zippers.

Parameters of the human genome.

  • N. Morton
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1991
Estimates of the physical and sex-specific genetic lengths are presented for each chromosome arm, with chiasma counts and linkage maps corrected for failure to sample telomeres giving an autosomal size of 2809 centimorgans in males and 4782 centim Morgans in females.