Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

  title={Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray},
  author={Mark Schena and Dari Shalon and Ronald W. Davis and Patrick O. Brown},
  pages={467 - 470}
A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential… 

Genome analysis with gene expression microarrays.

  • M. Schena
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1996
Mass-produced microarrays provide a new tool for genome expression analysis that may revolutionize genetic dissection, drug discovery and human disease diagnostics.

Preparation of mRNA for Expression Monitoring

In this unit, rationale for starting with poly(A+) versus total RNA is discussed, and strategies for choosing oligonucleotides for chip design is presented.

Preparation of mRNA for Expression Monitoring

In this unit, rationale for starting with poly(A+) vs. total RNA is discussed, and strategies for choosing oligonucleotides for chip design is presented.

Yeast microarrays for genome wide parallel genetic and gene expression analysis.

High-density DNA microarrays of yeast ORFs can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for sequence polymorphisms at the whole genome level.

Using array hybridization to monitor gene expression at the single cell level.

The protocol described has the potential of increasing the efficiency of tissue-specific expression analysis by combining high-throughput profiling with straightforward sampling and amplification procedures.

Analysis of Gene Expression

This review describes array manufacturing, experimental design, and its application to gene discovery and expression in DNA microarray technology, which is rapidly becoming a central platform for functional genomics.

Gene expression analysis using filter cDNA microarrays.

This chapter provides an overview of the methods necessary to carry out the production of membrane-based cDNA arrays and the subsequent synthesis and hybridization of radiolabeled cDNA probes.

Differential gene expression in Arabidopsis monitored using cDNA arrays.

This report studied the steady-state mRNA levels of over 800 Arabidopsis genes in parallel using high-density arrays of partially sequenced cDNA, which provides new functional information on anonymous genes and thus may guide reverse-genetics approaches.



Genes Galore: A Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones

The more than 13,400 plant ESTs that are currently available provide a new resource that will facilitate progress in many areas of plant biology and are illustrated by a description of the results obtained from analysis of 1500 Arabidopsis ESTs from a cDNA library prepared from equal portions of poly(A+) mRNA from etiolated seedlings, roots, leaves, and flowering inflorescence.

An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana.

As part of the goal to generate a detailed transcript map for Arabidopsis thaliana, 1152 single run sequences (expressed sequence tags or ESTs) have been determined from cDNA clones taken at random

Identification and map position of YAC clones comprising one-third of the Arabidopsis genome.

The results presented here represent a first step towards assembly of an overlapping YAC library of the A. thaliana genome.

Assessing mapping progress in the Human Genome Project.

Four measures that should be applied to any mapping project to describe its progress are outlined, believing that universal measures of mapping progress are both desirable and possible.

Arabidopsis thaliana and Plant Molecular Genetics

Arabidopsis thaliana is a small flowering plant with various properties that make it an excellent organism for experiments in molecular genetics. These properties include having a small nuclear

The human genome project. Prospects and implications for clinical medicine.

The recently initiated human genome project is a large international effort to elucidate the genetic architecture of the genomes of man and several model organisms and will have a profound impact on clinical medicine.

HD-Zip proteins: members of an Arabidopsis homeodomain protein superfamily.

  • M. SchenaR. W. Davis
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1992
It is concluded that Arabidopsis encodes a large family of homeodomain proteins, including members that contain a homeodOMain/leucine-zipper (HD-Zip) motif.

The HAT4 gene of Arabidopsis encodes a developmental regulator.

The HAT4 gene of Arabidopsis encodes an HD-Zip protein that functions as a novel developmental regulator that corresponds to highly conserved positions in known homeo domains and leucine zippers.