Quantitative FRET imaging of leptin receptor oligomerization kinetics in single cells.

  title={Quantitative FRET imaging of leptin receptor oligomerization kinetics in single cells.},
  author={Eva Biener and Madia Charlier and Vivek Ramanujan and Nathalie Daniel and Avital Eisenberg and Christian Bj\orbaek and Brian Herman and Arieh Gertler and Jean Djiane},
  journal={Biology of the cell},
  volume={97 12},
BACKGROUND INFORMATION Leptin, an adipocyte-secreted hormone, signals through activation of its membrane-embedded receptor (LEPR). To study the leptin-induced events occurring in short (LEPRa) and long (LEPRb) LEPRs in the cell membrane, by FRET (fluorescence resonance energy transfer) methodology, the respective receptors, tagged at their C-terminal with CFP (cyan fluorescent protein) or YFP (yellow fluorescent protein), were prepared. RESULTS The constructs encoding mLEPRa (mouse LEPRa)-YFP… CONTINUE READING

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