Quantitative Diffractometric Biosensing

@article{Blickenstorfer2020QuantitativeDB,
  title={Quantitative Diffractometric Biosensing},
  author={Yves Blickenstorfer and M. Muller and Roland Dreyfus and Andreas Michael Reichmuth and Christof Fattinger and Andreas Frutiger},
  journal={arXiv: Optics},
  year={2020}
}
Diffractometric biosensing is a promising technology to overcome critical limitations of refractometric biosensors, the dominant class of label-free optical transducers. These limitations manifest themselves by higher noise and drifts due to insufficient rejection of refractive index fluctuations caused by variation in temperature, solvent concentration, and most prominently, non-specific binding. Diffractometric biosensors overcome these limitations with inherent self-referencing on the… 

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References

SHOWING 1-10 OF 63 REFERENCES

Principles for Sensitive and Robust Biomolecular Interaction Analysis: The Limits of Detection and Resolution of Diffraction-Limited Focal Molography

Label-free biosensors enable the monitoring of biomolecular interactions in real-time, which is key to the analysis of the binding characteristics of biomolecules. While refractometric optical

Diffractive optics technology: a novel detection technology for immunoassays.

A novel diffractive optics technology (dot™) is detailed that takes advantage of the inherent properties of diffraction optics to deliver a cost-effective, portable, robust, optical biosensor that detects analytes at picomolar concentrations in complex media.

Diffractive Protein Gratings as Optically Active Transducers for High-Throughput Label-free Immunosensing.

A novel label-free biosensing approach based on bioreceptor networks patterned as diffractive gratings (biogratings) has been developed, reaching well-correlated responses with quantification and detection limits of 1.3 and 5.2 nM, respectively.

Large-scale resonance amplification of optical sensing of volatile compounds with chemoresponsive visible-region diffraction gratings.

The observed wavelength- and analyte-dependent amplification effects are quantitatively well described by a model that combines a Kramers-Kronig analysis with an effective-medium treatment of dielectric effects.

Optical biosensor assay (OBA).

A new biosensor immunoassay involving optical diffraction to detect clinically important analytes in human body fluids is described and demonstrated with a quantitative assay of choriogonadotropin in serum.

Optimizing the Limit of Detection of Waveguide-Based Interferometric Biosensor Devices

Waveguide-based photonic sensors provide a unique combination of high sensitivity, compact size and label-free, multiplexed operation. Interferometric configurations furthermore enable a simple,

Surface plasmon enhanced diffraction for label-free biosensing.

A diffraction biosensor to monitor the dynamic interaction of biological molecules in a label-free way was realized by the binding of an anti-biotin antibody to the biotin-functionalized region of a periodically patterned surface, which generated significant optical contrast to diffract the surface plasmon field.

Focal molography is a new method for the in situ analysis of molecular interactions in biological samples

The selective and sensitive detection of biomolecules, which bind to the recognition sites of the mologram in various complex biological samples, allows the label-free analysis of non-covalent interactions in complex samples, without a need for extensive sample preparation, and enables novel time- and cost-saving ways of performing and developing immunoassays for diagnostic tests.

Focal Molography: Coherent Microscopic Detection of Biomolecular Interaction

molecules in a liquid or gaseous sample may be deduced. The noncoherent surroundings of the coherently assembled molecules consist of freely diffusing solvent and solute molecules. The surroundings,
...