Quantitative Determination of Gap Junctional Permeability in the Lens Cortex

Abstract

We have developed a simple dye transfer method, which allows the gap junction permeability of lens fiber cells to be quantified. Two fixable fluorescent dyes (Lucifer yellow and rhodamine-dextran) were introduced into peripheral lens fiber cells via mechanical damage induced by removing the lens capsule. After a defined incubation period, lenses were fixed… (More)
DOI: 10.1007/s002329900521

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