Quantitative Determination of Eugenol from Cinnamomum tamala Nees and Eberm. Leaf Powder and Polyherbal Formulation Using Reverse Phase Liquid Chromatography

  title={Quantitative Determination of Eugenol from Cinnamomum tamala Nees and Eberm. Leaf Powder and Polyherbal Formulation Using Reverse Phase Liquid Chromatography},
  author={Vidya V. Dighe and Atish Gursale and Ramesh T. Sane and Sasikumar N. Menon and P. H. Patel},
A simple, rapid and precise reverse-phase high performance liquid chromatographic method has been developed for the quantitative determination of eugenol from the extract of dried powder of Cinnamomum tamala leaves and its polyherbal formulation. Chromatographic analysis was carried out on Zorbax C18 column (150 mm × 4.6 mm, 5 μm) with a mobile phase of mixture of water, acetonitrile and methanol in the volume ratio of 50:40:10, at a flow rate of 1.0 mL min-1. Quantitation was performed using a… 
Quantitation of Eugenol, Cinnamaldehyde and Isoeugenol from Cinnamomum tamala Nees and Eberm. Leaf Powder and Cinnamomum zeylanicum Breyn Stem Bark Powder by LC
An LC method has been developed for the simultaneous quantitative determination of eugenol, cinnamaldehyde and isoeugenol from dried leaf powder of Cinnamomum tamala Nees & Eberm and stem bark powder
Determination of eugenol in plants and pharmaceutical form by HPLC with amperometric detection at graphene-modified carbon paste electrode
Eugenol is an important natural compound derived from various plants, mainly in clove, and used for healthy benefits, cosmetics and as flavoring agent. A high-performance liquid chromatography (HPLC)
UV, HPLC Method development and quantification of eugenol isolated by preparative paper chromatography from Alcoholic extracts of different species of Ocimum
Around 2 ml of 99% eugenol was taken in a clean and dry test tube. The standard eugenol was spotted on a watt man filter paper having dimension 4 X 20 cm. 95% of methanol was mixed with 5% of
Development and validation of RP-HPLC-PDA method for the quantification of eugenol in developed nanoemulsion gel and nanoparticles
BackgroundEugenol is a potent phytochemical, and a plethora of delivery systems for this bioactive agent is being developed. Reversed-phase high-pressure liquid chromatography equipped with
SFE as a Superior Technique for Extraction of Eugenol-Rich Fraction from Cinnamomum tamala Nees (Bay Leaf) - Process Analysis and Phytochemical Characterization
Authors : Sudip Ghosh, Dipanwita Roy, Dipan Chatterjee, Paramita Bhattacharjee, Satadal Das Abstract : Highest yield of eugenol-rich fractions from Cinnamomum tamala (bay leaf) leaves were obtained
Physicochemical characterization and deodorant activity of essential oil recovered from Asiasarum heterotropoides using supercritical carbon dioxide and organic solvents
Abstract This study was aimed to extract oil from Asiasarum heterotropoides using supercritical carbon dioxide (Sc-CO2) with and without ethanol as co-solvent and to compare the results with
Chemical compositions of Cinnamomum tamala oil from two different regions of India
Abstract Objective This study was made to investigate the chemical composition of Cinnamomum tamala , (Buch.-Ham.) Nees & Eberm (Tejpat) oil (CTO) which was taken from two different regions. The
Simultaneous HPLC Determination of 22 Components of Essential Oils; Method Robustness with Experimental Design
A simple, precise and specific reversed phase HPLC method for the simultaneous determination of 22 components present in different essential oils namely cinnamon bark oil, caraway oil and cardamom fruit oil was developed and validated and demonstrated to be applicable to the determination of the ingredients in commercial sample of essential oil.
Determination of Eugenol in Aquatic Products by Dispersive Solid-Phase Extraction and Ultra-High-Performance Liquid Chromatography-Tandem Mass Spectrometry
A novel procedure, dispersive solid-phase extraction coupled with ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), was developed for the determination of eugenol
Ethnomedical, Pharmacological and Phytochemical Updates on Cinnamomum tamala
Cinnamomum tamala also known as Tezpat, having a place with the family Lauraceae, a noteworthy exposed tree in India, China, Nepal, Bhutan and Bangladesh. It is evergreen tree with medium in size.


Separation and Characterization of Pepper-Contaminated Cinnamon Using HPLC Analyses
Abstract A high performance liquid chromatographic method for the quantitation of cinnamaldehyde as well as trace amounts of eugenol and piperine in pepper-contaminated cinnamon was developed, using
Determination of estragole, safrole and eugenol methyl ether in food products
Abstract A rapid and effective procedure for the extraction and determination of 4-allylanisole (estragole), 4-allyl-1,3-benzodioxole (safrole) and 4-allyl-1,2-dimetoxybenzene (eugenol methyl ether)
Gas chromatographic determination of eugenol in ethanol extract of cloves.
An ethanolic extract of cloves was analyzed by gas chromatography directly to identify eugenol and other major phenolic compounds without previous separation of other components, and special attention was given to the major Phenolic compound, e Eugenol.
Chemical composition of essential oil of Cinnamomum tamala Nees et Eberm. leaves
A hydrodistilled oil obtained from the leaves of Cinnamomum tamala Nees et Eberm., grown in India, was analysed by capillary GC and GC–MS. The leaf volatile oil contained 40 constituents, of which a
Antitermitic activity of leaf essential oils and components from Cinnamomum osmophleum.
Investigation of the antitermitic activities of the essential oils from the leaves of two Cinnamomum osmophloeumclones and their chemical ingredients against Coptotermes formosanus Shiraki have demonstrated that the indigenous cinnamon B leaf essential oil has a more effective antiterMITic activity than indigenous cinnamon A leaf essentialOil.
Sensitive high-performance liquid chromatographic assay for the determination of eugenol in body fluids.
The high-performance liquid chromatographic assay described permitted a simple, rapid, sensitive, selective and precise quantitative determination of eugenol in body fluids without derivatization, suitable for pharmacokinetic studies.
Comparative evaluation of hypoglycaemic activity of some Indian medicinal plants in alloxan diabetic rats.
Present studies besides confirming hypoglycaemic activities of the experimental herbal samples, help identify more potent indigenous hyp glucosecaemic herbs (in crude ethanolic extract) from the comparative study of the reported experimental results.
Safety assessment of allylalkoxybenzene derivatives used as flavouring substances - methyl eugenol and estragole.
  • R. Smith, T. Adams, +9 authors I. Sipes
  • Chemistry, Medicine
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2002
Present exposure to methyl eugenol and estragole resulting from consumption of food, mainly spices and added as such, does not pose a significant cancer risk.
Practical HPLC method development
Getting Started. Basics of Separation. Detection Sensitivity and Selectivity. Sample Preparation. The Column. Non--Ionic Samples: Reversed-- and Normal--Phase HPLC. Ionic Samples: Reversed--Phase,
Indian Medicinal Plants
Indian medicinal plants / , Indian medicinal plants / , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی