Quantitative Colocalization Analysis of Multicolor Confocal Immunofluorescence Microscopy Images: Pushing Pixels to Explore Biological Phenomena

@article{Zinchuk2007QuantitativeCA,
  title={Quantitative Colocalization Analysis of Multicolor Confocal Immunofluorescence Microscopy Images: Pushing Pixels to Explore Biological Phenomena},
  author={Vadim S. Zinchuk and O. A. Zinchuk and Teruhiko Okada},
  journal={Acta Histochemica et Cytochemica},
  year={2007},
  volume={40},
  pages={101 - 111}
}
Quantitative colocalization analysis is an advanced digital imaging tool to examine antigens of interest in immunofluorescence images obtained using confocal microscopes. It employs specialized algorithms to estimate the degree of overlap of fluorescence signals and thus enables acquiring important new information not otherwise obtainable using qualitative approaches alone. As raw confocal images have high levels of background, they should be prepared to become suitable for reliable calculation… CONTINUE READING

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