Quantitative Analysis of Liposomal Heat Shock Protein 70 (Hsp70) in the Blood of Tumor Patients Using a Novel LipHsp70 ELISA

@article{Breuninger2014QuantitativeAO,
  title={Quantitative Analysis of Liposomal Heat Shock Protein 70 (Hsp70) in the Blood of Tumor Patients Using a Novel LipHsp70 ELISA},
  author={Stephanie Breuninger and Janina Erl and Clemens Knape and Sophie Gunther and Ivonne Regel and Franz R{\"o}del and Udo S. Gaipl and Jun Thorsteinsdottir and Lydia Giannitrapani and Anne M Dickinson and Gabriele Multhoff},
  journal={Journal of clinical \& cellular immunology},
  year={2014},
  volume={5},
  pages={1-10}
}
Elevated levels of the stress-inducible heat shock protein 70 (Hsp70) in the peripheral circulation have been reported for many tumor entities. In line with these findings we have shown that Hsp70 membrane-positive tumor cells actively release Hsp70 in exosome-like lipid vesicles. Since most commercial Hsp70 Enzyme-Linked Immunosorbent Assays (ELISAs) are not validated for the detection of liposomal Hsp70 in serum, the lipHsp70 ELISA was established using the monoclonal antibody cmHsp70.1 as a… 

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