Quantitative Analysis of Estrogen Receptor Proteins in Rat Mammary Gland.

@article{Saji2001QuantitativeAO,
  title={Quantitative Analysis of Estrogen Receptor Proteins in Rat Mammary Gland.},
  author={Shigehira Saji and Hideki Sakaguchi and Sandra E C Andersson and Margaret S. Warner and Jan-A Ke Gustafsson},
  journal={Endocrinology},
  year={2001},
  volume={142 7},
  pages={3177-3186}
}
Estrogen receptor a and b proteins (ERa and ERb) at various stages of development of the rat mammary gland were quantified by Western blotting. ERa and ERb recombinant proteins were used as standards, and their molar concentrations were measured by ligand binding assays. In 3-week-old pregnant, lactating, and postlactating rats the ERa content ranged from 0.30–1.55 fmol/mg total protein (mean values). The ERb content of the same samples ranged between 1.06–7.50 fmol/mg total protein. At every… CONTINUE READING

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