Quantitative Analyses of Circadian Gene Expression in Mammalian Cell Cultures

@article{Izumo2006QuantitativeAO,
  title={Quantitative Analyses of Circadian Gene Expression in Mammalian Cell Cultures},
  author={Mariko Izumo and Takashi R. Sato and Martin Straume and Carl Hirschie Johnson},
  journal={PLoS Computational Biology},
  year={2006},
  volume={2},
  pages={935 - 941}
}
The central circadian pacemaker is located in the hypothalamus of mammals, but essentially the same oscillating system operates in peripheral tissues and even in immortalized cell lines. Using luciferase reporters that allow automated monitoring of circadian gene expression in mammalian fibroblasts, we report the collection and analysis of precise rhythmic data from these cells. We use these methods to analyze signaling pathways of peripheral tissues by studying the responses of Rat-1… CONTINUE READING