Quantitative Amplification of Cleaved Ends (qACE) to assay miRNA-directed target cleavage [version 2; referees: 3 approved with reservations]


microRNA (miRNA) regulation is crucial to achieve precise spatio-temporal expression patterns of their target genes. This makes it crucial to determine the levels of cleavage of a particular target mRNA in different tissues and under different conditions. We developed a quantitative PCR method “quantitative Amplification of Cleaved Ends (qACE)” to assay… (More)


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